Hyperproduction of a bacterial mannanase and its application for production of bioactive mannooligosaccharides from agro-waste

Abstract

Bacillus subtilis MAN7 is known to produce a β-mannanase which is capable of producing mannooligosaccharides (MOS) from agro-wastes. Present study was aimed to hyper-produce the β-mannanase for its application in the cost-effective production of MOS from copra meal and evaluation of their bioactive properties. Optimization studies significantly enhanced the enzyme yield with an approximate 28.7fold increase. Enzyme was found to be remarkably stable under wide range of temperature and pH; making it suitable for the generation of MOS. Conditions were standardized for the pretreatment of copra meal which resulted in significant removal of undesirable components. MOS (DP2–7) was generated by the enzymatic hydrolysis of pretreated copra meal and found to exhibit effective prebiotic and antioxidant activity. Current study paves a way for the development of an eco-friendly and cost-effective bioprocess for the production of functional MOS from agro-waste using β-mannanase from B. subtilis MAN7.