Hyperpressure gas chromatography IV. Gas chromatography of etioporphyrin II metal chelates

Abstract

The hyperpressure gas chromatography of fifteen etioporphyrin II metal chelates of fourteen different elements has been studied at 145° and 1000–1700 p.s.i., with dichlorodifluoromethane as the solvent gas. The chelates of Sn(IV) and Mn(III) are insoluble under the experimental conditions and they are, thus, sharply separated from the other elements studied. A column of 10% Epon 1001 resin on Chromosorb W effected the following separations: Mg(II), Cu(II) or Co(II), Ni(II) or Co(III), VO or TiO, Mn(II), Zn or Pt(II) and Pd(II); Ag(II) Pt(II) and Pd(II). A 10% Silicone Gum Rubber XE-60 on Chromosorb W column separated Mn(II) or Fe(III), Zn or TiO, Pt(II) and VO. All the chelates were eluted intact with the exception of that of Mg(II), which was demetallated in the column. A novel method for the insertion of metal ions in the porphyrin ring, involving the reaction of the corresponding metal acetylacetonate with the porphyrin in a suitable solvent, is also reported.