Hyperpolarising Pyruvate through Signal Amplification by Reversible Exchange (SABRE).

Wissam Iali,Simon B Duckett,Soumya S Roy,Fadi Ahwal,Aneurin J Kennerley,Ben J Tickner

doi:10.1002/anie.201905483

Abstract

Hyperpolarisation methods that premagnetise agents such as pyruvate are currently receiving significant attention because they produce sensitivity gains that allow disease tracking and interrogation of cellular metabolism by magnetic resonance. Here, we communicate how signal amplification by reversible exchange (SABRE) can provide strong 13C pyruvate signal enhancements in seconds through the formation of the novel polarisation transfer catalyst [Ir(H)2(η2‐pyruvate)(DMSO)(IMes)]. By harnessing SABRE, strong signals for [1‐13C]‐ and [2‐13C]pyruvate in addition to a long‐lived singlet state in the [1,2‐13C2] form are readily created; the latter can be observed five minutes after the initial hyperpolarisation step. We also demonstrate how this development may help with future studies of chemical reactivity.

Highlights

Fig. S3: 1H NMR spectrum of the resulting methanol-d4solution formed under 3 bar of p-H2 and consisting of [IrCl(COD)(IMes)] precatalyst, 3 and DMSO at 298 K; hydride signals for the active species (equatorialbidentate form) dominate
We characterise the complex, [Ir(H)2(η2-pyruvate)(DMSO)(IMes)], that is formed during the SABRE process as illustrated in Scheme S1
The magnetic field B inside the shielded solenoid was measured relative to the applied voltage and current (I)

Summary

Introduction

In the former case, a solenoid coil is placed inside a mu-metal shield to achieve the desired matching field. Fig. S1: 1H NMR spectrum of a methanol-d4 solution under 3 bar H2 that contains the [IrCl(COD)(IMes)] precatalyst, DMSO and H2 at 245 K.

Results

Conclusion