Abstract
ObjectivesmiR-155 plays a critical role in the inflammatory process and in diseases such as rheumatoid arthritis (RA). miR155 gene expression is regulated by its gene promoter region CpG island methylation. Previous studies have shown inconsistent changes in circulating levels of mir-155 in RA patients. The aims of our study were to evaluate miR-155 levels in plasma, to investigate its gene methylation level, and to correlate these levels with RA disease activity.MethodsOne hundred and twenty-five patients with RA, and 30 age and sex-matched healthy controls (HC) were enrolled. Whole blood and plasma samples were collected and stored at -80°C until analysis. DAS28 score at the time of the blood draw was used to assess RA disease activity. The methylation status of miR-155 host gene was determined in whole blood by quantitative real-time methylation-specific PCR (qPCR). miR-155 expression levels were evaluated by quantitative reverse transcription PCR.ResultsWe found significantly lower circulating miR155 levels in RA patients compared to HC. Interestingly, the miR-155 gene methylation level was significantly higher in RA patients than in HC. miR-155 levels did not correlate with ACPA or RF positivity or disease activity.ConclusionsWe show here higher miR-155 methylation in whole blood and lower plasma miR155 expression in RA patients in comparison to HC. The evaluation of miR-155 host gene methylation status or miR155 plasma level might be a potentially useful marker in RA determination.
Highlights
The recognition of epigenetic mechanisms introduced a new aspect to understanding the etiopathogenesis of rheumatoid arthritis (RA)
We found significantly lower circulating miR155 levels in RA patients compared to healthy controls (HC)
The miR-155 gene methylation level was significantly higher in RA patients than in HC. miR-155 levels did not correlate with ACPA or RF positivity or disease activity
Summary
The recognition of epigenetic mechanisms introduced a new aspect to understanding the etiopathogenesis of RA. These mechanisms transmit signals from environmental factors to cell nuclei impacting gene expression [1]. The first are a wide group of nucleotide transcripts less than 200nt in length, and consist of micro-RNA (miRNA), small interfering RNA (siRNA), PIWI-interacting RNA (piRNA), small nucleolar RNA (snoRNA) and small nuclear RNA (snRNA). They play a fundamental role by regulating gene expression at the post-transcriptional level. They affect a wide range of developmental and cellular processes such as apoptosis, cell differentiation and proliferation, cellular migration, cell fate determination, pluripotency, neural plasticity and play a role in neurodegenerative disorders, cancers, infectious, and autoimmune diseases [5, 6]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.