Abstract
Hepatocellular carcinoma (HCC), the most common primary hepatic tumor, is highly prevalent in the Asia-Pacific region, including Thailand. Many genetic and epigenetic alterations in HCC have been elucidated. The aim of this study was to determine whether aberrant methylation of the suppressor of cytokine signaling 1 gene (SOCS1) occurs in HCCs. Methylation specific-PCR assays were performed to identify the methylation status of SOCS1 in 29 tumors and their corresponding normal liver tissues. An abnormal methylation status was detected in 17 (59%), with a higher prevalence of aberrant SOCS1 methylation significantly correlating with HCC treated without chemotherapy (OR=0.04, 95%CI=0.01-0.31; P=0.001). This study suggests that epigenetic aberrant SOCS1 methylation may be a predictive marker for HCC patients.
Highlights
Hepatocellular carcinoma (HCC) is the most common primary hepatic tumor and the fifth most common cancer in the world (McClune and Tong, 2010)
An abnormal methylation status was detected in 17 (59%), with a higher prevalence of aberrant suppressor of cytokine signaling 1 gene (SOCS1) methylation significantly correlating with HCC treated without chemotherapy (OR=0.04, 95%CI=0.01-0.31; P=0.001)
SOCS1 hypermethylation might be a key event for HCC transformation of cirrhotic nodules (Okochi et al, 2003) methylation of SOCS1 was more frequently observed in liver fibrosis, and HCV related-HCCs (Yang et al, 2003; Yoshida et al, 2004; Ko et al, 2008)
Summary
Hepatocellular carcinoma (HCC) is the most common primary hepatic tumor and the fifth most common cancer in the world (McClune and Tong, 2010). Pathogenic mechanisms in HCC include cirrhosis caused by alcoholic liver disease, metabolic influences, chronic hepatitis infection, and mutations occurring in single or multiple oncogenes or tumor suppressor genes (Whittaker et al, 2010), including epigenetic alterations. Epigenetic alterations, such as DNA methylation of CpG islands, associated with the transcriptional silencing of many genes, including tumor-suppressor genes, DNA repair genes, and metastatic inhibitor genes (Wong et al, 2001). The correlation between methylation status, SOCS1 expression level, and clinico-pathological features of the patients, were analyzed
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