Abstract
The present study has examined the effect of elevated glucose levels on membrane lipid peroxidation and osmotic fragility in human red blood cells (RBC). Defibrinated whole blood or RBC were incubated with varying concentrations of glucose at 37 degrees C for 24 h. RBC incubated with elevated levels of glucose showed a significantly increased membrane lipid peroxidation when compared with control RBC. A significant positive correlation was observed between the extent of glucose-induced membrane lipid peroxidation and the osmotic fragility of treated RBC. Glucose-induced membrane lipid peroxidation and osmotic fragility were blocked when RBC were pretreated with fluoride, an inhibitor of glucose metabolism; with vitamin E, an antioxidant; with para-chloromercurobenzoate and metyrapone, inhibitors of the cytochrome P-450 system; or with dimethylfurane, diphenylamine, and thiourea, scavengers of oxygen radicals. RBC treated with elevated glucose concentrations also showed an increase in NADPH levels. Exogenous addition of NADPH to normal RBC lysate induced membrane lipid peroxidation similar to that observed in the glucose-treated RBC. These data suggest that elevated glucose levels can cause the peroxidation of membrane lipids in human RBC.
Highlights
The present study has examined the effect of elevated peroxidation apparentlyinvolves NADPH andcytochrome P - glucose levels on membranelipid peroxidation and os- 450-like activity. motic fragility in human red blood cells (RBC)
Values for RBC treated with elevated levels ogf lucose and inhibitors are significantly lower than RBC treated with elevated levels of glucose without any inhibitor ( p
This study has demonstrated thealet vated levels of glucose fragility of RBC shows that the observedglucose-induced can result in the peroxidation of membrane lipids in RBC. lipid peroxidative damagecan cause changes in the properties
Summary
The molar ratio of serinetophosphorus and significant correlation between the glucose treatmentand ethanolamine to phosphorus was 0.40 and 0.47, respectively, phospholipid-MDA adduct (0.94 for RBC-PBS and 0.91 for and theTBA reactivity to phosphorus in the eluate of phosblood) or MDA It appears that the new lipid spot per ml of RBC and 0.92 and 0.87 when expressed per gof Hb, in glucose-treated RBC is formed by the cross-linking of PE, respectively). 0.8,28.6 f 1.3 (mean f S.D.) of RBC after treatmentof RBC- Fig. 2 shows that a similar new phospholipid can be PBS suspension with 5, 15, 25, 35, and 45 mM glucose This formed when normal RBC are treated in vitro with authentic shows that elevated glucose leveltreatment does not have any MDA. Percentage & 0.3, 2.9 f 0.8, 5.9 f 1.8, 12.2 -C 4.3, 17.7 f 6.1, and 24.1 f
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