Hyperglycaemia in pregnancy disturbs maternal-foetal well-being and alters placental function: role of inflammatory markers fkn and rank/rankl/opg system

Abstract

Event Abstract Back to Event Hyperglycaemia in pregnancy disturbs maternal-foetal well-being and alters placental function: role of inflammatory markers fkn and rank/rankl/opg system Noor E. Hashim1*, Mardhiah Tahir1, Intan S. Zulkafli1, Rasheeda Mohd. Zamin1 and Normadiah M Kassim1 1 University of Malaya, Malaysia Background Inflammation have been implicated for various diabetic complications. Fractalkine (FKN) and receptor activator of nuclear factor-kappaB ligand (RANKL)/RANK/osteoprotegerin (RANKL/RANK/OPG) systems are inflammatory markers suspected to be responsible for the changes seen in diabetic pregnancy. This study aimed to examine the effects of hyperglycaemia on pregnancy and investigate the role of these markers in the disease process. Methods Pregnant female Sprague-Dawley rats were injected with 45mg/kg BW of Streptozocin on gestational day (GD) 7 to induce diabetes followed by 10% glucose drink for 24h to prevent hypoglycaemic fatality. Control animals were injected with 1ml of citrate buffer vehicle. Diabetes was confirmed on GD15. Maternal weight and food intake were regularly monitored. Blood (maternal & foetal) and placenta separated into labyrinth and junctional zones were collected for morphological, biochemical and molecular analyses at GD15 and GD21. Results Maternal hyperglycaemia induced hyperphagia (p<0.05) and impeded maternal weight gain (p<0.05). Both groups were able to carry pregnancy to term but the hyperglycaemic group (HG) demonstrated trend towards increased foetal resorption at GD21 (13.6%, p=0.08) and 13.2% reduction of foetal weight (p<0.05). Although placenta weights were unchanged in all groups, feto-placental ratio dropped 15.8% in the HG group at GD21 (p<0.05). Morphologically, all placenta showed no significant differences in weight or thickness at both time points. Placenta zonal variations were observed in the mRNA expression of RANK, OPG, FKN and Vascular Endothelial Growth Factor A (VEGFa) but no hyperglycaemic effect was found. Conclusion This study demonstrated that maternal hyperglycaemia is detrimental in pregnancy through disruption of foetal survival (increased foetal resorption), disturbed maternal well-being (via altered eating pattern) and placental insufficiency through reduction of feto-placental ratio. Although expression of the inflammatory markers showed no significant role in the altered physiological milieu, more detailed studies are needed to delineate the exact role of these markers in hyperglycaemic pregnancy. Keywords: Maternal hyperglycaemia, Placenta, Pregnancy, Inflammatory markers, rat model Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Metabolic diseases Citation: Hashim NE, Tahir M, Zulkafli IS, Mohd. Zamin R and M Kassim N (2019). Hyperglycaemia in pregnancy disturbs maternal-foetal well-being and alters placental function: role of inflammatory markers fkn and rank/rankl/opg system. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00005 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Noor E Hashim, University of Malaya, Kuala Lumpur, Malaysia, nooreliza@um.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Noor E Hashim Mardhiah Tahir Intan S Zulkafli Rasheeda Mohd. Zamin Normadiah M Kassim Google Noor E Hashim Mardhiah Tahir Intan S Zulkafli Rasheeda Mohd. Zamin Normadiah M Kassim Google Scholar Noor E Hashim Mardhiah Tahir Intan S Zulkafli Rasheeda Mohd. Zamin Normadiah M Kassim PubMed Noor E Hashim Mardhiah Tahir Intan S Zulkafli Rasheeda Mohd. Zamin Normadiah M Kassim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.