Abstract
Porous graphitic carbon HPLC packing material developed by John Knox’s research group and commercialised as Hypercarb™ has been available on the market for over thirty years. However, the unique properties of this phase still prove to be fundamentally novel and provide solutions to difficult separation applications. Of these properties, its lack of an elutropic series and hence ability to provide good retention not just in reversed-phase HPLC but even with non-aqueous solvents was put to good effect in this work. Herein it was demonstrated, the resolution of permethrin isomers, the isolation of coal tar extract constituents, the separation of a homologous series of garlic oil constituents, the clean-up of plant extract fractions and the provision of orthogonal selectivity for the separation of flurbiprofen and its related substances.
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