Abstract
A series of novel hyperbranched poly[2-(α-d-mannopyranosyloxy) ethyl methacrylate-co-N,N’-methylenebisacrylamide] (HPManEMA-co-MBA) are synthesized via a reversible addition fragmentation polymerization (RAFT). The dosage ratios of linear and branch units are tuned to obtain different degree of branching (DB) in hyperbranched glycopolymers. The DB values are calculated according to the content of nitrogen, which are facilely determined by elemental analysis. The lectin-binding properties of HPManEMA-co-MBA to concanavalin A (ConA) are examined using a turbidimetric assay. The influence of defined DB value and molecular weight of HPManEMA-co-MBA on the clustering rate is studied. Notably, HPManEMA-co-MBAs display a low cytotoxicity in the MTT assay, thus are potential candidates for biomedical applications.
Highlights
Carbohydrates and their corresponding conjugated glycoproteins are key participants in a measureless array of biological functions, and can be used as the carrier or drug in the treatment of diseases, such as cancer, cytotoxic chemotherapy or radiotherapy [1,2,3,4,5,6,7,8]
Hyperbranched glycopolymers HPAcManEMA-co-MBA was synthesized via reversible addition-fragmentation chain transfer polymerization (RAFT) polymerization
In the chain growth stage, the RAFT polymerization mechanism illustrated that CTA reacted with chain radicals, and the new formation lost the ability to initiate vinyl groups
Summary
Carbohydrates and their corresponding conjugated glycoproteins are key participants in a measureless array of biological functions, and can be used as the carrier or drug in the treatment of diseases, such as cancer, cytotoxic chemotherapy or radiotherapy [1,2,3,4,5,6,7,8]. Polysaccharides being of high molecular weight show strong affinity due to the “cluster glycoside effect” [15,16,17,18]. It should be noted that the DB value played an important role in the recognition event Technologies, such as nuclear magnetic resonance (NMR), viscosity measurements and light-scattering methods, are often used to evaluate the DB value of hyperbranched polymers [30,31,32,33]. Aware of the importance of “cluster glycoside effect”, it would be highly desired to prepare hyperbranched glycopolymers whose DB values can be determined using a facile method. The DB values of the hyperbranched glycopolymers were facilely determined via elemental analysis because the nitrogen atoms are merely contained in branch units. The resulting hyperbranched glycopolymers were studied by MTT assay to test their cytotoxicity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
