Hyperbilirubinemia models caused by hemolysis in newborn rhesus monkeys

Abstract

Objective To establish the newborn rhesus monkey model of hemolytic hyperbilirubinemia and provide an experimental basic model for research of hyperbilirubinemia. Methods Sixteen 3-day old newborn rhesus monkeys were divided into experimental group and control group, with 8 newborn rhesus monkeys in each group.Eight newborn rhesus monkeys in experimental group were treated with intravenous injection of 10 g/L phenylhydrazine hydrochloride(50 mg/kg) to establish model of homolytic hyperbilirubinemia.The newborn rhesus monkeys in control group were treated with intravenous injection of 9 g/L saline at the same time.Twenty-four hours and 48 hours after the experimental treatment, the bilirubin in blood was detected to evaluate the models, and the clinical manifestations of newborn rhesus monkeys with hyperbilirubinemia were recorded by using monitoring equipment.The brain slices were made to evaluate the model in 1 dead monkeys of experimental group. Results The newborn rhesus monkey of experimental group showed obvious skin, sclera jaundice and hemoglobinuria.The serum total bilirubin [(252.76±63.42) μmol/L], unconjugated bilirubin[(165.85±44.93) μmol/L] and conjugated bilirubin [(87.16±21.22) μmol/L] in the experimental group were significantly higher than those [(20.62±5.72) μmol/L, (7.93±2.31) μmol/L, (12.51±3.53) μmol/L] in the control group, and the differences were statistically significant (t=14.581, 13.881, 14.040, all P<0.01). The level of hemoglobin [(47.18±10.09) μmol/L] in the experimental group was significantly lower than that of the control group [(136.85±13.48) μmol/L], and the difference was statistically significant (t=-21.308, P<0.01). The results of pathological showed brain edema, rupture and eosinophilic and bilirubin deposition in the basal nuclei, and necrosis appeared in some severe parts.And there were different degrees of retardation and coordination disorders in the experimental group's newborn rhesus monkeys, but gradually returned to normal in 4 months later. Conclusion Intravenous injection of phenylhydrazine hydrochloride can be used to produce newborn rhesus monkey models of hemolytic hyperbilirubinemia. Key words: Hyperbilirubinemia; Phenylhydrazine hydrochloride; Hemolysis; Animal model