Hyperbetalipoproteinemia with small low-density lipoprotein, a characteristic disorder of lipoprotein in essential hypertension

Abstract

The purpose of the present study was to elucidate the characteristic lipoprotein disorder in essential hypertension. Twenty-six patients with essential hypertension (HT) but without diabetes mellitus or obesity and 24 healthy subjects (control) were recruited into this study. Lipoproteins of HT and controls were separated by ultracentrifugation to very-low-density lipoprotein (VLDL), intermediate density lipoprotein (IDL), low-density liproprotein (LDL), and (HDL) fractions. Cholesterol and triglycerides were determined with enzyme assay, and apoB were determined by highly sensitive latex agglutination (Kyowa-hakko Co. LD). There was no difference in age (mean ± SE; HT, 63 ± 2 versus control, 60 ± 2 years) or body-mass index (22.7 ± 0.4 versus 21.7 ± 0.5 kg/m 2) between HT and controls. Blood pressure in HT and controls was 158 ± 2/87 ± 12 mm Hg and 123 ± 3/72 ± 2 mm Hg, respectively. Cholesterol did not change significantly in plasma (192.1 ± 7.0 versus 176.4 ± 4.2 mg/dL), VLDL (15.2 ± 2.4 versus 11.8 ± 1.7 mg/dL), IDL (14.8 ± 2.4 versus 10.7 ± 1.6 mg/dL), LDL (93.7 ± 4.6 versus 83.1 ± 3.9 mg/dL), nor in HDL (51.9 ± 2.7 versus 58.1 ± 3.2 mg/dL). Triglycerides (TG) increased in plasma (120.0 ± 10.0 versus 87.5 ± 9.3 mg/dL, p < 0.05), although TG did not change in all subfractions. ApoB increased in plasma (105.5 ± 5.1 versus 85.6 ± 3.6 mg/dL, p < 0.01), IDL (9.0 ± 1.3 versus 5.4 ± 0.6 mg/dL, p < 0.05), and LDL (76.3 ± 4.3 versus 59.4 ± 3.7 mg/dL, p < 0.01) in HT compared with controls. The ratio of cholesterol to apoB in LDL decreased (1.27 ± 0.06 versus 1.48 ± 0.08, p < 0.05). In essential HT, number of apoB containing lipoproteins (IDL, LDL) increased. Low ratio of cholesterol to apoB was noted in LDL, indicating the presence of small, dense LDL. As cholesterol in LDL was normal, hyperbetalipoproteinemia is also a characteristic disorder of essential HT.