Hyperbaric oxygen therapy positively interferes with oxidative metabolism in female cats undergoing vídeo-assisted elective ovariohysterectomy

Abstract

This study aimed to determine whether hyperbaric oxygen therapy (HBOT) alters oxidative biomarkers after elective ovariohysterectomy (OHE). For this purpose, 45 healthy female cats were randomized into three groups: the hyperbaric group (HG): 15 animals pretreated with HBOT and submitted to OHE; the hyperbaric control group (HCG): 15 animals pretreated with HBOT without surgery; and the sham group (SHAM): 15 female cats submitted to OHE without pretreatment. The following biomarkers were evaluated: superoxide dismutase (SOD) and catalase (CAT), reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE). The collection times were: T1 = before the surgery for the operated groups and after sedation in the HCG; T2 = 30 min after reversal of sedation (HCG) or at the time of extubation in the other groups; T3 = 24 hr after T2. There was no difference regarding serum dosage of ROS, AChE, or BChE. There was an increase in TBARS in the T3 SHAM compared to the T3 HG (p=0.043). Moreover, CAT activity decreased in T2 SHAM and T3 SHAM compared to T1 SHAM (p=0.012 and p<0.001mauric); T2 SHAM had lower CAT activity than T2 HCG (p=0.05). Additionally, the T3 SHAM was lower than the T3 HG (p=0.030) and T3 HCG (p=0.050). There was reduced SOD in the T2 SHAM compared to the T2 HG (p=0.033) and T2 HCG (p=0.027). Similarly, the T3 SHAM decreased compared to the T3 HG (p=0.039) and T3 HCG (p=0.019).