Abstract
To investigate the consequences of the association between hyperbaric oxygen therapy and hepatic ischemia / reperfusion. Wistar rats were divided into three groups: SHAM, rats submitted to surgical stress and anesthetic but not hepatic ischemia or reperfusion, I / R, rats submitted to total hepatic pedicle ischemia for 30 min, followed by 5 min of reperfusion; HBO120, rats submitted to 120 min of hyperbaric oxygen therapy at two absolute atmospheres and immediately after submitted to the experimental protocol of ischemia and reperfusion. The preservation of the hepatic function was evaluated by determining mitochondrial swelling and malondialdehyde tissue level, as well as alanine aminotransferase and aspartate aminotranferase serum levels. The results were analyzed using the Mann-Whitney test and differences were considered significant for p<0.05. There were significant differences in values: mitochondrial swelling of the I / R group compared to SHAM and HBO120; malondialdehyde between SHAM vs. I / R, SHAM vs HBO120, and I / R vs HBO120, alanine aminotransferase between SHAM vs. I / R . There was no significant difference between groups in aspartate aminotransferase serum levels. The association between hyperbaric oxygen therapy and hepatic ischemia and reperfusion process was positive.
Highlights
Hyperbaric oxygen therapy is widely used in various contexts involving, especially, diseases related to phenomena of hypoxia, ischemia and reperfusion[1,2] there is no scientific consensus on the subject; there are works in favor[3,4] and unfavorable[5] to this association
In order to determine whether exposure to hyperbaric oxygen therapy is harmful when applied before hepatic exposure to a process of ischemia and reperfusion, the analysis of mitochondrial swelling was adopted as a parameter of hepatic functional preservation because it is widely used in several literature works in this area[8,9]
Twenty four male Wistar rats weighing between 200 to 300g were divided into three groups: SHAM - mice submitted to surgical stress and anesthesia, without exposure to hyperbaric oxygen therapy (HBO) and without hepatic pedicle clamping, I / R - mice submitted to 30 min of liver ischemia by clamping the hepatic pedicle, followed by 5 min of reperfusion without HBO exposure; HBO120 – rats submitted to 120 min of HBO at two absolute atmospheres (ATA) and immediately underwent 30 min of ischemia followed by 5 min of reperfusion
Summary
Hyperbaric oxygen therapy is widely used in various contexts involving, especially, diseases related to phenomena of hypoxia, ischemia and reperfusion[1,2] there is no scientific consensus on the subject; there are works in favor[3,4] and unfavorable[5] to this association. This phenomenon is due, at least in part, to a lack of studies that explore the biochemical basis of this association[6,7], precisely the proposal of this work. Malondialdehyde tissue level (MDA) was taken as parameters of hepatic lesion for it is a recognized marker of lipid peroxidation[10,11] as well as serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) considered as indicators of hepatocellular integrity with an association between increased serum levels of these enzymes and acute hepatic damage[12,13,14,15]
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