Abstract
Background: Hyperbaric Oxygen (HBO) Therapy improves the outcome of various types of sugery, such as postoperative bone grafts, fixation of jaw fractures, and osteoplasty of jaw deformities. Therefore, it is important to determine the effects of HBO on bone regeneration. The purpose of this study was to clarify the influence of a hyperbaric oxygen environment on bone regeneration at the osteoclastogenic cytokines level. Sample & Methods: RAW264.7 cells were stimulated under the various conditions by using a hyperbaric oxygen chamber. We evaluated the ability of the RAW264.7 macrophages to proliferate, differentiate and produce various osteoclastogenic cyto-kines. Results: A hyperbaric oxygen (HBO) environment and high concentration oxygen (HCO) environment increase cellular proliferation in a time-dependent manner. On the other hand, a HCO environment and a hyperbaric with room air (HBA) environment enhanced the differentiation of RAW264.7 cells. In addition, NFATc1 and c-Fos were increased by both the HBA environment and HCO environment. However, the effects of HBA and HCO environments were in contrast with each other with regard to RANK, TNF-α, C-FMS and TRAP. Conclusions: It was suggested that a HBO environment influenced the bone regeneration by altering osteoclastogenic cytokines level, and that a HCO environment and HBA environment acted independently on the proliferation and differentiation of macrophages and the secretion of osteoclastogenic cytokines, and that they acted in concert in a hyperbaric oxygen environment to induce conditions favoring regeneration.
Highlights
Hyperbaric Oxygen (HBO) Therapy improves the outcome of various types of surgery, such as the repair of bone fractures [1]-[3], osteoplasty of jaw deformities, the treatment of osteoradionecrosis [4]-[6] and distraction osteogenesis [7] [8], as well as postoperative bone grafting [1] [9] and the use of dental implants [10]
We examined the effects of HBO on RAW264.7 cell proliferation using the MTS assay
Under hyperbaric with room air (HBA) conditions, the proliferation was significantly lower for the cells treated for 20 minutes × 3, but under the other conditions, no differences in proliferation were noted between the treated cells and untreated controls (Figure 2(b))
Summary
Hyperbaric Oxygen (HBO) Therapy improves the outcome of various types of surgery, such as the repair of bone fractures [1]-[3], osteoplasty of jaw deformities, the treatment of osteoradionecrosis [4]-[6] and distraction osteogenesis [7] [8], as well as postoperative bone grafting [1] [9] and the use of dental implants [10]. The reason was generally assumed that HBO stimulates the growth of blood vessels, resulting in an increased blood supply and enhanced bone formation [11]. This is partly because HBO is believed to generate reactive oxygen species (ROS) and reactive nitrogen species (RNS) that affect signaling processes critical to wound healing. Conclusions: It was suggested that a HBO environment influenced the bone regeneration by altering osteoclastogenic cytokines level, and that a HCO environment and HBA environment acted independently on the proliferation and differentiation of macrophages and the secretion of osteoclastogenic cytokines, and that they acted in concert in a hyperbaric oxygen environment to induce conditions favoring regeneration
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
