Hymenobacter citatus sp. nov., isolated from soil in South Korea.

Abstract

Two novel Gram-negative bacterial strains, BT507T and BT506, were isolated from soil collected in Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains BT507T and BT506 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae; order Cytophagales; class Cytophagia). The 16S rRNA genes of the two strains shared a sequence similarity of 100.0%. Strains BT507T and BT506 are closely related to Hymenobacter profundi M2T (97.2% 16S rRNA gene similarity), Hymenobacter defluvii POA9T (97.1%), and Hymenobacter tenuis POB6T (95.3%). The genome size of strain BT507T is 5,078,289 base pairs. Bacterial growth was observed at 10-37°C (optimum 25°C) and pH 6.0-8.0 (optimum pH 6.0). The primary cellular fatty acids of strain BT507T are iso-C15:0, C16:1ω5c, and summed feature 3 (C16:1ω6c/C16:1ω7c). Its predominant respiratory quinone is MK-7. The primary polar lipids of strain BT507T are lipid, aminophospholipid, and phosphatidylethanolamine. Based on the biochemical, chemotaxonomic, and phylogenetic analysis, strains BT507T and BT506 can be described a novel bacterial species within the genus Hymenobacter, and the proposed name is Hymenobacter citatus. The type strain of H. citatus is BT507T is KCTC 82115T and NBRC 114850T.