Hydroxysafflor yellow a the protective effect of endothelial cells induced by LPC

Abstract

The current study showed that safflower yellow pigment can protect the endothelial cells, reduce the incidence of coronary restenosis, but the specific mechanisms have not been reported. Objective: To establish in vitro hemolytic blood lecithin-induced endothelial cell models of restenosis and of safflower yellow pigment on the vascular endothelial cell protection. Methods Cultured human umbilical vein endothelial cells (HUVECs), divided into: (1) normal control group, (2) lysophosphatidylcholine (LPC) group, (3) low concentrations of safflower yellow + lysolecithin group (HYSA: 50mg / L + LPC), (4) high concentrations of safflower yellow + lysolecithin group (HYSA: 100mg / L + LPC) (each group interventions? group name full uniform). Were observed endothelial cell proliferation, apoptosis, and supernatant nitric oxide (NO) concentration changes. Results and Conclusion: Compared with normal control group, lysophosphatidylcholine inhibit endothelial cell proliferation, promoting apoptosis, reducing the NO concentration, the difference was significant (P <;0.01). Safflower yellow pigment intervention lysophosphatidylcholine on endothelial cells, the enhancement of endothelial cell proliferation, decreased apoptosis, NO concentration, with the normal control group and LPC group difference was significant (P <;0.01). The results confirmed that the safflower yellow pigment can be improved LPC effects on HUVECs, enhanced epithelial cell proliferation, decreased apoptosis, increased NO concentration.