Hydroxypropyl methyl cellulose-Satureja hortensis L. ethanol extract mixtures as antimicrobial coating for sutures, identification of phenolic acids by using LC-MS-MS and TLC techniques

Abstract

In this study, phenolic acid content of Satureja hortensis L. ethanol extract was investigated using TLC and LC-MS-MS methods and thymol, vanillic acid and caffeic acid were identified. Antimicrobial activity of prepared Hydroxypropyl methylcellulose (HPMC)-plant ethanol extract mixture was screened against E. coli (ATCC 25922), S. aureus (ATCC 25923) and B. cereus (ATCC 14579) species by using TLC-bioautography, disk and well diffusion methods. Both plant extract and the extract-biopolymer mixture showed inhibition activity on Gram-positive species according to TLC-bioautography results. Considering the disk diffusion test results, it was found that the inhibition activity increased with biopolymer plant extract mixtures for Gram-positive bacteria. Finally, the combination of plant extract with HPMC was used as coating for surgical sutures and antimicrobial activities were performed by using agar diffusion method. All coated sutures showed antimicrobial activity against S. aureus. Absorbable coated suture also exhibited antimicrobial activity against Bacillus cereus.