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Abstract
Abstract The AOAC method for riboflavin determination has been modified to improve the precision of the manual method and greatly simplify its automation. In the AOAC method, permanganate oxidation is used for sample cleanup, and hydrogen peroxide is used to reduce excess permanganate. By substituting hydroxylamine. HCl for the hydrogen peroxide, the generation of oxygen gas, which can be troublesome, is eliminated without otherwise changing the assay. The resulting manual assay has a relative standard deviation of 1% as opposed to 2.5% for the same assay with hydrogen peroxide. This method is simple to automate, using a continuous flow system. The automated system performs 40 determinations per hour with a 1% relative standard deviation and results agree with those from the AOAC method at the 98% confidence level.
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