Abstract
The mutagenicity of hydroxylamine (HA) has been tested in 8 different adenine-requiring mutants ( ad-3B) of Nuerospora crassa: 4 mutants of this tester set revert after treatment with nitrous acid and ethyl methanesulfonate indicating that they revert by a base-pair substitution, 2 mutants revert only after treatment with ICR-170 indicating that they revert by a base-pair insertion or deletion, and 2 mutants revert only spontaneously. HA induced reversions in 3 of the 4 mutants which revert by base-pair substitution and in none of the others. The specificity of the action of HA on the mutants in the present tester set is consistent with the hypothesis that the predominant class of genetic alterations induced by HA in Neurospora is base-pair transitions from guanine-cytosine to adenine-thymine. Furthermore, it was found that the reversion frequency after HA treatment increases in proportion to the square of the treatment time.
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