Hydroxyl Radical-Scavenging Activity-Guided Extraction of Peptides from Oviductus ranae

Jieyang Shen,Lixue Zheng,Yihui Liu,Yang Zhang,Jiahao Xu,Xiaoxiao Xu,Shenbo Ye,Yang Liu

doi:10.3844/ajbbsp.2021.361.372

Jieyang Shen, Lixue Zheng + Show 6 more

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https://doi.org/10.3844/ajbbsp.2021.361.372

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Abstract

Hydroxyl Radical-Scavenging Activity-Guided Extraction of Peptides from <i>Oviductus ranae</i>

Highlights

Reactive Oxygen Species (ROS), including a variety of unstable free radicals are inevitable products of oxidative metabolism (Kurniawan et al, 2020)
In single-factor experiment, the hydroxyl radical-scavenging rate of Antioxidant Peptides from OR (APOR) was at first elevated with the increase of enzyme amount and incubation time, decreased with the further increase of enzyme amount and incubation time (Figs 1 ~ 4), which could be attributed to the saturability and negative feedback of enzymes (Yin et al, 2018)
APOR obtained by the above-mentioned optimal parameters comprise seventeen amino acids with total content of 581.3 mg.g-1, which was higher than that of Oviductus Ranae (OR) (426.8 mg.g-1) (Zhang et al, 2017), but lower than that of Proteins from OR (POR) (668 mg.g-1) (Zhang et al, 2019b). These results indicated that enzymolysis could promote the release of amino acids from OR powders, leading to a relatively higher amino acids content of APOR than that of untreated counterpart

Summary

Introduction

Reactive Oxygen Species (ROS), including a variety of unstable free radicals are inevitable products of oxidative metabolism (Kurniawan et al, 2020). These free radicals are widely involved in the regulation of physiological and biochemical functions. Many protein-based antioxidants are limited by the macromolecular structures that hinder the interaction with free radicals (Xia et al, 2012). It is a feasible and common way to obtain peptides with antioxidant potentials by hydrolyzing maternal proteins by different proteases (Zhao, 2018). Studies have confirmed that antioxidant peptides can potentiate the therapeutic effects of non-peptide antioxidants (De Luca et al, 2016; Schroeder et al, 2005)

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