Abstract
Flower formation in stem explants was chosen as an experimental system for the study of the function of hydroxycinnamoyl amides (HCAs) in plants. The explants, derived from flowering and non‐flowering Nicotiana tabacum L. var. Xanthi nc., differentiated to two types of callus and afterwards to flower buds. A novel reversed‐phase high performance liquid chromatography method, following sample clean‐up on CM‐Fractogel columns, enabled us to examine HCA concentrations in small tissue samples. Two different groups of HCA could be distinguished during in vitro flower formation: Firstly, feruloyl‐ and diferuloyputrescine, the major detectable HCAs that accumulated during callus proliferation; secondly, caffeoylputrescine, which accumulated during the later stage of flower differentiation and reached higher concentrations than feruloylputrescine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
