Hydroxycinnamic acids in the raw material of hybrid bearded iris

Abstract

Representatives of Iris genus ( Iridaceae ) are widespread in almost all continents. According to the literature data, Iris species accumulate secondary metabolites such as flavonoids, isoflavonoids, xanthones, coumarins, hydroxycinnamic acids, saponins, tannins, vitamins, organic acids. The aim of this work was the establishment of the qualitative and quantitative composition of hydroxycinnamic acids in the raw materials of iris hybrid varieties, as well as to establish the antimicrobial activity of the dry extracts and isolation of individual compounds. Materials and methods. The objects of study were the leaves and rhizomes of the standard dwarf bearded iris (SDB). The substances were isolated by column chromatography, nature was established by physical and physicochemical methods of analysis (UV-, IR spectroscopy, TLC). Antibacterial activity was determined by the agar well diffusion method in vitro . Results. Hydroxycinnamic acids were identified in the iris raw materials by paper chromatography. The spectrophotometric method established of the hydroxycinnamic acid content in the recalculation on chlorogenic acid – was from 0.79 % to 2.83 %. The dry extracts of the leaves and rhizomes of I. hybrida “Little Dream” at a 1 % concentration have shown moderate inhibitory activity for Gram-positive bacteria and fungi. For the first time from the rhizomes of Iris hybrida “Indian Pow Waw” were isolated caffeic, chlorogenic, neochlorogenic, ferulic acids by the column chromatography and their structures were determined by spectral methods. Conclusions. The content of hydroxycinnamic acids in the samples varies from 0.79 % to 2.83 %. The antimicrobial activity of the dry extracts of I. hybrida “Little Dream” leaves and rhizomes was established. For the first time from the rhizomes of Iris hybrida “Indian Pow Waw” were isolated 4 derivatives of hydroxycinnamic acid. The results of the study show the prospects for the use of cultivated varieties of irises as a source of hydroxycinnamic acids.