Abstract
Hydroxyapatite is widely used clinically in the field of dentistry, however, the modulating action of hydroxyapatite with respect to the cells has not been investigated. In this study, it is described that one of the cytokines released by fibroblasts can cause increasing alkaline phosphatase (ALPase) activity of fibroblastsin vitro. Human pulp fibroblasts (HPFs) were cultured with hydroxyapatite (30–480 µg cm−2) in serum-supplemented medium for 6 to 96 h, then the serum-free conditioned medium was obtained and added to sub-cnfluent HPF cultures. Significantly enhanced ALPase activity was detected when HPFs were incubated with the conditioned medium. This HPF-derived activity which induces increasing ALPase activity appeared in the conditioned medium with a 12h culture period, and the appearance of this mediator was completely inhibited in the presence of cycloheximide. Enhanced ALPase activity is detected in lesions in which calcification is occurring. The results of this experiment suggest that HPFs in contact with hydroxyapatite serve as a mediator in cell-to-cell interaction, and are related to the functional differentiation of cells for tissue repair including pulpal calcification.
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