Hydroxyapatite mineralization of chitosan-tragacanth blend/ZnO/Ag nanocomposite films with enhanced antibacterial activity

Abstract

Biocompatible nanocomposites (NCs) with antibacterial activity containing organic matrix and inorganic nanoparticles (NPs) are vital for providing a suitable substrate for hydroxyapatite (HA) formation. Therefore, we fabricated a series of biocompatible NCs of chitosan (CS) and tragacanth gum (TG) and different percentages of ZnO NPs and ZnO@Ag NPs as fillers into the CS-TG blend. The characteristics of the NCs were distinguished with the field-emission scanning electron microscope (FE-SEM), X-Ray diffraction, Fourier transform infrared, and transmission electron microscopy (TEM). The CS-TG/ZnO@Ag(1:0.500) NC 8 wt% showed a rough surface according to FE-SEM. Moreover, the TEM image of CS-TG/ZnO NC 8 wt% depicted a uniform dispersion of NPs into the matrix. The biocompatibility of these NCs was evaluated by the formation of HA on their surfaces. The outcomes depicted the deposition of HA on the surface of all NCs. Also, CS-TG/ZnO@Ag(1:0.500) NC 8 wt% exhibited the most HA deposition on its surface. The antibacterial activity of these NCs toward Staphylococcus aureus and Escherichia coli bacteria was evaluated. The CS-TG/ZnO@Ag(1:0.500) NC 8 wt% exhibited a higher inhibition zone diameter in comparison to the ZnO@Ag (1:0.500) NPs for the S. aureus bacteria. Generally, antibacterial activity of the NCs containing ZnO@Ag NPs are more than NCs containing ZnO NPs.