Abstract
Basic experiments were carried out to explore whether an organic phosphate, Calcium β-glycerophosphate (Ca-BGP), could be used as the medicament for direct pulp capping. Synthetic incu-bation medium nearly matched the exposed surface of the dental pulp. Crystal formation in this mediumby supplementation of serum and Ca-BGP was ultrastructually evaluated. After 3 hours of incubation, numerous globular structures, consisting of small granules were formed. These globular structures were identified as amorphous calcium phosphate (ACP) by electron diffraction and IR analyses. With time, globular structures decreased and needle-like crystals increased. Needle-like crystals were identified as hydroxyapatite (HAP) by electron diffraction. Alkaline phosphatase (ALP) activity in the medium with Ca-BGP decreased for up to 12 hours of incubation, and after that time, equilibrium occurred. These findings suggest that supplemented Ca-BGP is hydrolyzed by seru m ALP in the synthetic incu-b ation medium. Also the ACP is preferentially produced in high Ca X inorga nic phosphate (Pi) ion-product medium and finally ACP converts to thermodynamically stable HAP. Then, Ca-BGP could be used as new agent for direct pulp capping.
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