Hydroxyapatite Biosynthesis Obtained from Sea Urchin Spines (Strongylocentrotus purpuratus): Effect of Synthesis Temperature

Nayeli Sarahi Gómez Vázquez,Alfredo Rafael Vilchis Nestor,Claudia Mariana Gomez Gutierrez,Manuel De Jesús Chinchillas Chinchillas,Ruben Cesar Villarreal Sánchez,Priscy Alfredo Luque Morales,Osvaldo De Jesus Nava Olivas

doi:10.3390/pr8040486

Nayeli Sarahi Gómez Vázquez, Alfredo Rafael Vilchis Nestor + Show 5 more

Open Access

https://doi.org/10.3390/pr8040486

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Journal: Processes	Publication Date: Apr 22, 2020
Citations: 5	License type: CC BY 4.0

Affiliation: Autonomous University of Baja California

Abstract

In this investigation, hydroxyapatite (HA) was synthesized using sea urchin spines (Strongylocentrotus purpuratus) via a precipitation and heat treatment method at three different temperatures (500, 600 and 700 °C). Biosynthesized HA was characterized to determine the vibration of functional groups, morphology, particle size, crystalline structure and chemical composition. For this, Fourier-Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR), Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X-ray Spectroscopy (EDS), X-ray Diffraction (XRD) and X-ray Photoelectron Spectroscopy (XPS) were used, respectively. The FTIR-ATR results reveal that the most defined characteristic HA bonds (O-H, P-O and C-O bonds) were better defined at higher synthesis temperatures. SEM also presented evidence that temperature has a significant effect on morphology. EDS results showed that the Ca/P ratio increased in the samples at higher temperatures. XRD analysis presented the characteristic peaks of HA, showing a lower crystallinity when the synthesis temperature increased. Finally, the XPS confirmed that the material resulting from biosynthesis was HA. Hence, according to these results, the synthesis temperature of HA has a significant effect on the characteristics of the resulting material.

Highlights

Hydroxyapatite (HA), an important crystalline material in the medical field, is composed of calcium, phosphorus and hydrogen atoms (Ca10 (PO4 )6 (HO)2 ) and generally has a hexagonal crystalline structure [1]
For the CaO synthesis, the sea urchin spines were washed with distilled water until the organic
There are no vibrations of the organic contamination bonds observed in the FTIR-ATR spectrum, so these results indicate high-purity HA

Summary

Introduction

Hydroxyapatite (HA), an important crystalline material in the medical field, is composed of calcium, phosphorus and hydrogen atoms (Ca10 (PO4 ) (HO)2 ) and generally has a hexagonal crystalline structure [1] This material is present in teeth and bones, presenting a characteristic hardness and, due to its chemical and physical properties, has the application of facilitating the regeneration of hard tissues [2]. HA has been used in sensors in liquid and gaseous environments [7], in the treatment of wastewater to adsorb dyes [8] and as a coating on metallic body implants [9], among other uses This last application stems from the need to repair large bone defects and avoid the use of autografts and allografts, which often involve limitations such as rejection rate, insufficient donors or a high risk of disease transmission [10]

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