Abstract
Abstract The utility of hydroxy naphthol blue (HNB) as a spectrophotometric and fluorometric reagent for the uranyl ion has been investigated. In phthalate buffer (at a pH of 4.0), UO2 2+ forms a brown complex of low absorptivity with the red form of HNB. By following the decrease in HNB absorbance at 530 nm (which has e = 4.1 × 103) uranyl ion can be determined to levels as low as 1.1 × 10−6 M (0.30 μg/ml). HNB also emits at 460 nm when excited at 365 nm at these pH values, while the UO2 2+ complex exhibits greatly reduced emission. Examination of the quenching of HNB emission by UO2 2+ allows the determination of uranyl ion to levels as low as 3.2 × 10−6 M (0.86 μg/ml). A 1:1 type complex was formed between UO2 2+ and HNB, and a formation constant of 9.77 × 103 (log K1 = 3.99) was measured for the complex.
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