Abstract
Dark fermentation (DF) for hydrogen (H2) evolution is often limited to industrial application due to its low H2 yield. In this work, hydrothermal carbon microspheres (HCM) and iron modified HCM (Fe-HCM) were prepared by hydrothermal process using waste corn cob. Subsequently, HCM and Fe-HCM were used in DF for more H2. The highest H2 yields amended with HCM and Fe-HCM at 600 mg/L were achieved to be 119 and 154 mL/g glucose (0.87 and 1.2 mol H2/mol glucose), respectively, being 24% and 59% higher than that of control yield. Soluble metabolites revealed HCM and Fe-HCM promoted butyric acid-based DF. Microbial composition depicted that HCM and Fe-HCM improved the abundance level of Firmicutes from 35% to 41% and 56%, while the abundance level of Clostridium_sensu_stricto_1 rose from 25% to 38% and 51%, respectively. This provides valuable guidance for hydrothermal carbon used in biofuel production.
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