Abstract
Kale (Brassica oleracea var. alboglabra L.) seedlings were selected for cultivation as Se-enriched vegetable by a hydroponic system. Se-enriched kale seedlings were grown in Hoagland's solution supplemented with 5, 10, 15, 30 and 45μgmL−1 Se (IV) from sodium selenite for 15days and harvested every 5days. Cultivation of kale seedlings with 45μgmL−1 Se inhibited the growth rate and Se accumulation. However, total Se concentrations of all Se-supplemented treatments were higher than that of control treatment. The highest Se concentration accumulated by kale approximately 400μgg−1 Se (expressed as Se in dry matter) was obtained in the kale which was grown with 30μgmL−1 Se in solution. Se Speciation studies indicated that the predominant forms of organic Se are selenomethionine (SeMet), Se-methylselenocysteine (SeMC) and unknown which were identified in 0.1M HCl in 10% methanol extracts by ion pairing reversed phase HPLC–ICP-MS. Moreover, almost 100% of Se in the extract was found to be non-metabolized selenite when harvested longer than 15days. Thus, it is possible to produce Se-enriched seedling with the method proposed.
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