Abstract
Phenylalanine-specific transfer RNA from methionine-starved relaxed Escherichia coli K12 separates into two components when chromatographed on Octyl-Sepharose. The difference in elution between the two tRNAs has been shown to depend on the methyl group in the highly modified 2-methylthio-N-6-isopentenyladenosine. The first eluted tRNA Phe lacks this methyl group, while the last eluted tRNA Phe is fully methylated. Other differences in the modification patterns have no effect on the elution from Octyl-Sepharose. The elution pattern of tyrosine- and serine-specific tRNAs, also normally containing ms 2i 6 A, is similar.
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