Hydrophobic attachment of Trypanosoma cruzi to a superficial layer of the rectal cuticle in the bug Triatoma infestans.

Abstract

In the vector Triatoma infestans the human pathogenic flagellate Trypanosoma cruzi colonizes mainly the rectum. The rectal cuticle and associated trypanosomes were examined by electron microscopy. The cuticle of the rectum consisted of a superficial wax layer, which was not retained by conventional preparation, an outer and inner epicuticle, and a procuticle. Epimastigotes of T. cruzi attached to the superficial layer with a specialized area of the flagellum. The composition of the cuticle was analyzed by cytochemistry to determine constituents relevant for parasite attachment. Intense staining with the fluorochrome Nile red indicated the presence of lipids, and measurements of contact angles formed by test fluids with the rectal wall revealed that the luminal surface is hydrophobic. The mechanism of attachment of T. cruzi was found to be based on a hydrophobic interaction. The flagellates bound to lipids extracted from the cuticle and to saturated hydrocarbons. Chitin, which has been presumed to be the natural binding substrate of T. cruzi, was localized using gold-labeled wheat-germ lectin. Chitin occurred in the procuticle but was absent from the superficial and epicuticular layers and, thus, is not accessible for binding by T. cruzi. In addition, it could not be confirmed that galactose-specific lectins or heparin receptors mediate flagellate attachment to the rectum.