Hydrolysis of sugar nucleotides in chicken egg white in response to embryonic development.

Abstract

The egg white of newly laid chicken egg was found to contain about 45 mumol of UDP-N-acetylgalactosamine 4-sulfate, 34 mumole of GDP-mannose, 6 mumol of UDP-N-acetylhexosamine, and 1 mumol of UDP-N-acetylgalactosamine 4,6-bissulfate per liter. There was no significant difference between infertile and fertile eggs in the initial levels of the sugar nucleotides. During incubation for 4 days, the nucleotide levels in infertile eggs showed little change while those in fertile eggs fell continuously until the complete disappearance of the nucleotides on the fourth day. Initial removal of the blastoderm from fertile eggs resulted in cessation of the reduction in nucleotide levels in the 2- to 4-day period after the operation. The decrease of UDP-N-acetylgalactosamine 4-sulfate was followed by an increase of 1-phospho-N-acetylgalactosamine 4-sulfate then N-acetylgalactosamine 4-sulfate in the egg white. When UDP-N-acetylgalactosamine 4-[35S]sulfate or GDP-[14C]mannose was injected into the egg white of a fertile egg, the main feature of the metabolism of the labeled compounds was the successive hydrolysis of their pyrophosphate and phosphate bonds, with the formation of sugar 1-phosphate and sugar. A significant activity of nucleotide pyrophosphatase was detected in the egg white in newly laid eggs (0-day egg). However, no such activity could be detected in egg-white specimens from 1-, 2-, and 3-day eggs. The results suggest that although the decrease of sugar nucleotides in the first day could be ascribed to the hydrolytic action of the enzyme originally present in the egg white, the decrease in the subsequent 3 days results from a more complex process in which the hydrolysis of the sugar nucleotides is related to the development of the embryo.