Abstract

The activity of enzymes releasing glucose and reducing sugars from sucrose, maltose, starch and dextran was compared in the same pooled samples of plaque fluid (PF) from 24 h human dental plaque. Equimolar amounts of glucose and fructose were released from sucrose in 3 h incubations. Reducing activity was released from sucrose or starch at a similar rate. The rate of glucose release from the starch substrate was similar to that from maltose but lower than that from sucrose. Raffinose was hydrolysed, indicating β-fructosidase activity in PF. The hydrolysis of maltose, trehalose and melezitose confirmed the presence of α-glucosidase activity. Maltose was metabolized partially to a maltosaccharide. No dextranase activity was detectable in PF, and the soluble polymeric carbohydrate in PF was partially degraded by fungal dextranase. Starch was degraded to dextrins, maltose and glucose.

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