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Abstract
The genus of Termitomyces purchased from the market has been identified as Termitomyces eurrhizus using the Internal Transcribed Spacer (ITS) method. An α-galactosidase from T. eurrhizus (TEG), a monomeric protein with a molecular mass of 72 kDa, was purified 146 fold by employing ion exchange chromatography and gel filtration. The optimum pH and temperature was 5.0 and 60 °C, respectively. TEG was stable over pH 2–6, and also exhibited good thermostablility, retaining 100% of the original activity after incubation at 60 °C for 2 h. Inhibition of the enzyme activity by N-bromosuccinimide (NBS) constituted evidence for an essential role of tryptophan in the catalytic action of the isolated enzyme. Besides 4-nitro-phenyl α-d-galactophyranoside (pNPGal), natural substrates could also be effectively hydrolyzed by TEG. Results of thin-layer chromatography (TLC) revealed complete enzymatic hydrolysis of raffinose and stachyose to galactose at 50 °C within 6 h. These properties of TEG advocate its utilization for elevating the nutritional value of soymilk.
Highlights
Different mushrooms of the genus Termitomyces like Termitomyces striatus, T. eurrhizus, Termitomyces microcarpus, Termitomyces robustus and Termitomyces clypeatus, which grow abundantly in termite nests, have been identified as wild edible mushrooms with high nutritive value
Α-Galactosidases (EC 3.2.1.22) are widely distributed in microorganisms [6,7,8], plants [9] and mammals. These enzymes catalyse the hydrolytic removal of α-1,6-linked α-galactose residues from simple oligosaccharides including melibiose, raffinose, stachyose and polymeric galactomannans (Figure 1) which are employed in the sugar industry to improve sucrose crystallization by hydrolysing raffinose in beet sugar syrups
They are mainly used in the sugar industry, where they improve the crystallization of sucrose by hydrolysing the raffinose in beet sugar syrups [10], used in the foInot.dJ
Summary
Different mushrooms of the genus Termitomyces like Termitomyces striatus, T. eurrhizus, Termitomyces microcarpus, Termitomyces robustus and Termitomyces clypeatus, which grow abundantly in termite nests, have been identified as wild edible mushrooms with high nutritive value. Α-Galactosidases (EC 3.2.1.22) are widely distributed in microorganisms [6,7,8], plants [9] and mammals These enzymes catalyse the hydrolytic removal of α-1,6-linked α-galactose residues from simple oligosaccharides including melibiose, raffinose, stachyose and polymeric galactomannans (Figure 1) which are employed in the sugar industry to improve sucrose crystallization by hydrolysing raffinose in beet sugar syrups. They are mainly used in the sugar industry, where they improve the crystallization of sucrose by hydrolysing the raffinose in beet sugar syrups [10], used in the foInot.dJ. We describe an α-galactosidase isolated from Termitomyces eurrhizus which has. TahlaiscteonsziydmaesehaissgoolaotdetdhefrrmomostaTbeirlimtyitaonmdyacceidsiceustrarbhiilzituys, awndhich has been idaelnsotifiexeidhibuistsinhgigthhheyIdTroSlymtiectahcotidvi.tyTthoiws aerndzsyomligeoshaaccshgaorioddest. hermostability and acidic stability, and exi2h.iRbeitssulhtsigh hydrolytic activity towards oligosaccharides
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