Abstract
Direct observations of the enzymatic hydrolysis of C10 acyclic allylic isoprenyl diphosphates by an acid phosphatase from the leaves ofCinnamomum camphora (camphor tree) were made using1H and31P NMR spectrometers. The measurements indicated that the allylic primary diphosphates, geranyl diphosphate and neryl diphosphate, were hydrolyzed to their corresponding alcohols in a sequential manner via their corresponding monophosphates, whereas the allylic tertiary diphosphate, linalyl diphosphate, was hydrolyzed only to its corresponding monophosphate.
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