Hydrolysis and absorption of proline dipeptides across the wall of sacs prepared from everted rat intestine

Abstract

1. 1. Transfer of intact glycol- l-proline, l-prolylglycine, l-prolyl- l-proline, l-leucyl- l-proline, l-prolyl- l-hydroxyproline and N- formyl- l-leucine to the interior of sacs prepared from 5 cm segments of everted rat intestine was observed. The amounts varied from 0.24 ± 0.15 (S.E.) μmole per 30 min for leucylproline to 2.54 ± 0.23 μmole per 30 min for prolylproline. Of the two dipeptides containing glycine and proline there was significantly more transfer of prolylglycine than of glycylproline. 2. 2. For most of the compounds examined, an inverse relationship was found between the intact dipeptide or N-formylleucine transferred in 30 min and the total hydrolysis in the same period.