Hydrogenation of biomass pyrolysis oil using enhanced reduction catalytic capacity cellular by expressing excavated carbonyl reductase

Abstract

A biocatalytic method to achieve the hydromodification of biomass fast pyrolysis oil was implemented. Two carbonyl reductases SDR-1 and SDR-8 were excavated in the genome of Bacillus coagulans NL01, enhanced Escherichia coli ability to tolerate and reduce various toxic ketone aldehydes. 10 g/L furfural and 8 g/L acetoin as bio-oil model compounds could be transformed by the recombinant E. coli SDR-1 and E. coli SDR-8 respectively. The biphasic catalysis system was constructed to convert the keto-aldehyde compounds in bio-oil to the corresponding alcohol at 30 °C. Recombinant E. coli SDR-8 could completely consume 20 g/L glucose and consume 60% 20 g/L glucose in the diluted 50-fold and 30-fold bio-oil catalytic system, respectively. This provided a new pathway for the hydrogenation of bio-oil by biocatalytic method and laid the foundation for the low-temperature esterification in the later stage.