Abstract
We constructed three hydrogenase mutants from Anabaena sp. PCC 7120: Δ hupL (deficient in uptake hydrogenase), Δ hoxH (deficient in bidirectional hydrogenase), and Δ hupL/Δ hoxH (deficient in both genes), and showed that the Δ hup and Δ hupL/Δ hoxH produced H 2 at a rate 4–7 times that of wild-type under optimal conditions (Appl. Microbiol. Biotechnol. 58 (2002) 618). We have studied H 2 producing activity of Δ hup in more detail. H 2 producing activity of Δ hup cells was moderately improved in older cultures when 1% CO 2 was added to the bubbling air. The efficiency of light energy conversion to H 2 by the Δ hupL mutant at its highest H 2 production stage was 1.0–1.6% at an actinic visible light intensity of lower than 50 W/ m 2 under argon atmosphere, and the activity lasted for at least 35 min . At 250 W/ m 2 , H 2 producing activity gradually decreased with illumination time.
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