Abstract

Strains of Zymomonas mobilis grown in media containing either glucose or sucrose were assessed for the production of hydrogen sulphide (H 2S). In a liquid medium with low glucose concentration (20 g l −1) only a proportion of the strains tested formed H 2S, but in medium containing a higher glucose concentration (100 g l −1) all the strains tested produced H 2S. Four Z. mobilis strains were assayed quantitatively for H 2S production and strain ZM4 was found to produce the most H 2S in glucose medium. The amount of yeast extract and glucose, and the type of sugar used in the medium affected the amount of H 2S formed by strain ZM4. A mutant, designated ZM4701, of strain ZM4 was isolated which did not produce any detectable H 2S in liquid medium containing yeast extract plus either glucose or sucrose. The nutritional requirements of ZM4701 were investigated.

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