Abstract
This study investigated the biological removal of hydrogen sulfide (H2S) from biogas by anaerobic wastewater treatment processes. Heterotrophic and autotrophic bacteria were isolated from upflow anaerobic sludge blanket (UASB)-mixed liquor of fruit processing factory using nutrient broth and thiosulfate mineral salt medium, respectively. Hydrogen sulfide-removal activities were investigated using sterile UASB-mixed liquor and hydrogen sulfide as growth substrates. It was found that the growth rate of autotrophic bacteria (319 mg VSS l−1 day−1) was higher than that of the heterotrophic bacteria (267 mg VSS l−1 day−1). Hydrogen sulfide(g) was totally removed by absorption into liquid phase: 78·24% removal of aqueous sulfide (S2−(aq)) was achieved by autotrophs and 68·03% by heterotrophs. Molecular identification by polymerase chain reaction-denaturing gradient gel electrophoresis technique and DNA sequencing showed that heterotrophic consortium consisted of Pseudomonas fluorescens Pf0-1, P. aeruginosa PAO1, P. putida KT2440, Bacillus megaterium DSM319 and B. cereus ATCC14579. Autotrophic consortium consisted of Thiobacillus thioparus NZ, T. denitrificans ATCC25259 and Halothiobacillus neapolitanus c2.
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