Abstract
Raw albacore tuna was separated into water-soluble and water-insoluble fractions. The water-soluble fraction was separated into two fractions by trichloroacetic acid (2.5%). The water-insoluble fraction was extracted with NaCl (0.45 M), and then the insoluble fraction was further extracted with urea solution (6 M). H2S production upon heating of each fraction was determined by the reflux-trap technique. Water-soluble proteins produced 71% of the H2S, while the corresponding contribution from the insoluble fraction was 29%. Non-protein soluble components and salt-soluble proteins produced insignificant amounts of H2S. Significant increase in the amount of H2S was observed when water-insoluble proteins were treated with urea and TCA solutions.
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