Hydrogen Sulfide Prevents Mesenteric Adipose Tissue Damage, Endothelial Dysfunction, and Redox Imbalance From High Fructose Diet-Induced Injury in Aged Rats.

Oleh Revenko,Yaroslav Pavlovskiy,Vasyl Kovalyshyn,Olena Varyvoda,Maryana Savytska,Ilona Chelpanova,Oksana Zayachkivska,Antonina Yashchenko

doi:10.3389/fphar.2021.693100

Oleh Revenko, Yaroslav Pavlovskiy + Show 6 more

Open Access

https://doi.org/10.3389/fphar.2021.693100

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Abstract

A high fructose diet (HFD) and advanced age are key factors for the gradual loss of physiological integrity of adipose tissue. Endogenous hydrogen sulfide (H2S) has beneficial effects on cytoprotection and redox balance. But its interactive effects on age-related damage of mesenteric vessels and connective and adipose tissues (MA) during HFD which could be the base of the development of effective physiological-based therapeutic strategy are unknown. The aim of study was to investigate age- and HFD-induced mesenteric cellular changes and activities of enzymes in H2S synthesis and to test the effects of sodium hydrosulfide (NaHS) which is considered an H2S donor on them. Adult and aged male rats on a standard diet (SD) or 4-week HFD were exposed to acute water-immersion restraint stress (WIRS) for evaluation of mesenteric subcellular and cellular adaptive responses by electron microscopy. The effects of exogenous NaHS (5.6 mg/kg/day for 9 days) versus vehicle on mesentery changes were investigated. Serum glucose level, thiobarbituric acid reactive substances (TBARS), and activities of cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), thiosulfate-dithiol sulfurtransferase (TST), and sulfite oxidase (SO) were examined by spectrophotometry. In both adult and aged SD groups, treatment with NaHS protected mesenteric cells after WIRS. In both groups, the treatment with NaHS also protected MA mitochondria, microvascular endothelial and sub-endothelial structures, and fibroblasts versus the vehicle-treated group that had signs of damage. HFD increased MA injury and mitochondrial changes in both aged and adult rats. HFD-associated malfunction is characterized by low activities of CSE, CBS, TST, SO, and increased TBARS. Finally, we demonstrated that pretreatment with NaHS inhibited MA and mitochondria alterations in aged rats exposed to HFD and WIRS, lowered TBARS, and enhanced H2S enzyme activities in contrast to the vehicle-treated group. Mitochondrial integrity alterations, endothelial damage, and redox imbalance are key factors for rat mesenteric adipose tissue damage during advanced age. These alterations and MA hypertrophic changes retain the central for HFD-induced damage. Moreover, H2S signaling contributes to MA and mitochondria redox balance that is crucial for advanced age and HFD injury. The future study of H2S donors’ effects on mesenteric cells is fundamental to define novel therapeutic strategies against metabolic changes.

Highlights

There is growing evidence that suggests the importance of the functional roles of adipose tissue
After 4 weeks of high fructose diet (HFD) administration in adult animals, fasting glucose levels were increased by 25% (p < 0.05) versus adults on standard diet (SD); no differences were noted between the vehicle group and supplemented sodium hydrosulfide (NaHS)
We found that treatment by NaHS in adult rats exposed to stress on HFD resulted in increased activities of cystathionine beta-synthase (CBS)–16%, cystathionine gamma-lyase (CSE)–37%, and thiosulfate-dithiol sulfurtransferase (TST) by 5% over adult rats which did not receive NaHS (p < 0.01) and aged rats, CBS–28%, CSE–51%, and TST–13% (p < 0.01)

Summary

Introduction

There is growing evidence that suggests the importance of the functional roles of adipose tissue This includes white adipocytes, brown adipocytes, and beige adipocytes, which differ in morphology and functions (Hilton et al, 2015; Shimizu et al, 2015; Lee et al, 2019). These cells are unique in their ability to collect and integrate thousands of different types of input and to translate them into signaling pathways that are responsible for pleiotropic expression contributing to the risk of numerous metabolic disorders related to obesity, type 2 diabetes, and gastrointestinal and cardiovascular diseases processes (Li et al, 2018; Scheja et al, 2019; Zhu et al, 2019). The link between mesenteric white adipocytes damage during aging and the chronic overload nutrition of glycemic carbohydrates still remains incomplete; in this report, we demonstrate that mesenteric cells can change in advanced age and have compared their change during HFD

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