Abstract
Hydrogen sulfide (H<sub>2</sub>S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H<sub>2</sub>S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H<sub>2</sub>S levels, the H<sub>2</sub>S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in <em>Vitis vinifera</em> L. ‘F-242’. Furthermore, the seedlings were treated with sodium hydrosulfide (NaHS, a H<sub>2</sub>S donor) and hypotaurine (HT, a H<sub>2</sub>S scavenger) at 4°C to examine the effects of exogenous H<sub>2</sub>S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of <em>VvICE1</em> and <em>VvCBF3</em> genes, but low level of super oxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H<sub>2</sub>S might be directly involved in the cold signal transduction pathway of grape.
Highlights
Hydrogen sulfide (H2S) is a novel gasotransmitter along with nitric oxide (NO) and carbon monoxide (CO) [1]
It is reported that exogenous H2S can enhance the resistance of plants to drought stress [11,12,13,14] and copper stress [15,16] by improving the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)
Our results showed that H2S was involved in cold stress response in grape by regulating superoxide anion radical content, MDA content, the relative permeability of cell membrane and SOD activity, as well as expression of VvICE1 and VvCBF3
Summary
Hydrogen sulfide (H2S) is a novel gasotransmitter along with nitric oxide (NO) and carbon monoxide (CO) [1]. Recent research indicated that multiple transcription factors and inducers, such as C-repeat-binding factor (CBF), inducers of CBF expression (ICE) and cold-regulated (COR). Our results showed that H2S was involved in cold stress response in grape by regulating superoxide anion radical content, MDA content, the relative permeability of cell membrane and SOD activity, as well as expression of VvICE1 and VvCBF3
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