Hydrogen Sulfide (H₂S) Releasing Capacity of Isothiocyanates from Moringa oleifera Lam.

Xiangshe Wang,Yi Lin,Xingdi Liu,Xueqin Zheng,Yunjiao Liu,Yuyun Lu

doi:10.3390/molecules23112809

Abstract

Moringa oleifera Lam. is rich in phytochemical compounds especially glucosinolates (GSs) and isothiocyanates (ITCs), which are active compounds for cancer chemoprevention benefits of Brassicaceae vegetables. In this study, we determined the total contents of GSs and ITCs and their specific profiles in different Moringa tissues including seeds, stems, leaves and roots. Seeds (seeds with shell and seed kernel) showed significantly higher levels of total GSs and ITCs than that of other Moringa tissues. The hydrogen sulfide (H2S) releasing capacity of total ITCs extracted from different Moringa tissues was determined by lead (II) acetate assay in 24-well plates. The H2S releasing capacity of different Moringa tissues were evaluated and compared. Moringa seeds showed the highest H2S releasing capacity, followed by roots, leaves and stems. Our results suggest that Moringa based foods may exhibit health benefits due to its GSs and ITCs contents that are the precursors for H2S, in addition to the recognized action mechanisms of ITCs.

Highlights

Hydrogen sulfide (H2 S), with the characteristic foul odour of rotten egg, has been regarded as a new pleiotropic endogenous gasotransmitter besides nitric oxide (NO) and carbon monoxide (CO).Numerous studies showed that it might be beneficial to human health in neuroprotection, antioxidant, anticancer and blood vessel relaxation [1,2,3,4]
H2 S is mainly endogenously produced from L-cysteine under the catalysis of related enzymes including cysteine aminotransferase (CAT), 3-mercaptopyruvate sulphur transferase (3-MST), cystathionine β-synthase (SBC) and cystathionine γ-lyase (CSE) [7]
The aim of this study is to investigate the H2 S releasing possibility and compare the H2 S releasing capacity of

Summary

Introduction

Hydrogen sulfide (H2 S), with the characteristic foul odour of rotten egg, has been regarded as a new pleiotropic endogenous gasotransmitter besides nitric oxide (NO) and carbon monoxide (CO). Numerous studies showed that it might be beneficial to human health in neuroprotection, antioxidant, anticancer and blood vessel relaxation [1,2,3,4]. A previous study reported that H2 S plays a key role in regulating cardiovascular homeostasis, acting as a direct relaxing regent in the vascular smooth muscle [5]. H2 S is mainly endogenously produced from L-cysteine under the catalysis of related enzymes including cysteine aminotransferase (CAT), 3-mercaptopyruvate sulphur transferase (3-MST), cystathionine β-synthase (SBC) and cystathionine γ-lyase (CSE) [7]. The benefits of endogenous H2 S in human health highlight its great usefulness in the functional regulation.

Objectives

Methods

Results

Conclusion