Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Zhu-Lin Luo,Jian-Dong Ren,Fu-Zhou Tian,Ke Xiang,Tao Wang,Hao-Tong Xu,Chen Fang,Long Cheng,Li-Jun Tang

doi:10.1155/2015/281985

Abstract

Purpose. This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats. Methods. Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines. Results. Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants. Conclusion. Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

Highlights

In recent years, pancreas transplantation has become the treatment of choice for patients with insulin-dependent diabetes mellitus [1,2,3]
The administration of hydrogen-rich saline partially restored the level of insulin in the heterotopic pancreas transplantations (HPT) + HS group and caused a noticeable increase compared with the HPT + NS group
Hydrogen-rich saline administration significantly reduced the amylase and lipase levels in the HPT + HS group compared with those in the HPT + NS group (P < 0.05). These results indicated that the hydrogen-rich saline might be helpful for the recovery of endocrine function of pancreas and it reduced the severity of graft pancreatitis

Summary

Introduction

Pancreas transplantation has become the treatment of choice for patients with insulin-dependent diabetes mellitus [1,2,3]. Graft pancreatitis as a result of ischemia/reperfusion (I/R) injury is one of the most severe complications in the early postoperative period [4]. In cases of graft in other tissues, oxygen free radicals cause direct membrane damage, expression of adhesion molecules, and neutrophil infiltration, which mediate the damage. Many factors lead to the generation of oxygen-derived free radicals during the reperfusion period, including endothelial dysfunction, endogenous enzymes, and leucocyte recruitment [6]. Postimplantation pancreatitis induced by I/R might be caused or promoted by a prooxidative and an antioxidative imbalance. This theory led to the development of a new therapeutic strategy for reducing

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