Abstract
BackgroundIt is well considered that reactive oxygen species (ROS) plays a prominent causative role in the development of allergic rhinitis (AR), and eosinophils cells as important allergic inflammatory cells contribute to elevating oxidative stress. Hydrogen, emerging as a novel antioxidant, has been proven effective in selectively reducing ROS in animals models of oxidative damage. We herein aim to verify protective effects of hydrogen on eosinophils cells in guinea pigs models of AR.MethodsThirty two guinea pigs were random divided into four groups, and AR model was established through ovalbumin sensitization. The guinea pigs were injected with hydrogen-rich saline (Normal-HRS and AR-HRS group) or normal saline (control and AR group). The frequencies of sneezing and scratching were recorded. The IgE level, blood eosinophil count and eosinophil cationic protein (ECP) level in serum were measured. The serum malondialdehyde (MDA) and superoxide dismutase (SOD) assays were also measured to evaluate oxidative stress. The expression levels of eotaxin mRNA and protein in the nasal mucosa were also determined by real-time RT-PCR, Western blot and immunofluorescence.ResultsHRS reduced the ROS and MDA levels and increased SOD level in guinea pigs of AR-HRS group accompanied with decreased frequency of sneezing and scratches. Meanwhile, there was a decline of the number of eosinophils cells in blood and of thelevel of ECP in serum in the AR-HRS group. HRS also significantly decreased the expression of eotaxin in nasal mucosa.ConclusionHRS may play a protective role in attenuating allergic inflammation, and suppressing the increase and activation of eosinophils in AR possibly through antioxidation effect of hydrogen.
Highlights
It is well considered that reactive oxygen species (ROS) plays a prominent causative role in the development of allergic rhinitis (AR), and eosinophils cells as important allergic inflammatory cells contribute to elevating oxidative stress
Recent studies have shown that oxidative stress and the production of reactive oxygen species (ROS) contribute to allergic inflammation, such as asthma and AR
The AR models were prepared as follows. 32 guinea pigs were randomly divided into four groups (n = 8 each group), namely, Control groups, normal-HRS, AR-NS and AR-HRS
Summary
It is well considered that reactive oxygen species (ROS) plays a prominent causative role in the development of allergic rhinitis (AR), and eosinophils cells as important allergic inflammatory cells contribute to elevating oxidative stress. Especially allergic rhinitis (AR), is a major health problem. Several treatments such as corticosteroid and anti-histamine drugs are available for dealing with it, their side effects have limited the use of them. Recent studies have shown that oxidative stress and the production of reactive oxygen species (ROS) contribute to allergic inflammation, such as asthma and AR. Many observations suggest that oxidative stress plays an important role in the pathogenesis of airway allergic inflammation such as asthma and AR [3].
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