Abstract
Hydrogen (H2), a new antioxidant, was reported to reduce •OH and ONOO− selectively and inhibit certain proinflammatory mediators to product, without disturbing metabolic redox reactions or ROS involved in cell signaling. We herein aim to explore its protective effects on acute renal injury in sodium taurocholate-induced acute pancreatitis and its possible mechanisms. Rats were injected with hydrogen-rich saline (HRS group) or normal saline (SO and SAP group) through tail intravenously (6 mL/kg) and compensated subcutaneously (20 mL/kg) after successful modeling. Results showed that hydrogen-rich saline attenuated the following: (1) serum Cr and BUN, (2) pancreatic and renal pathological injuries, (3) renal MDA, (4) renal MPO, (5) serum IL-1β, IL-6, and renal TNF-α, HMGB1, and (6) tyrosine nitration, IκB degradation, and NF-κB activation in renal tissues. In addition, it increased the level of IL-10 and SOD activity in renal tissues. These results proved that hydrogen-rich saline attenuates acute renal injury in sodium taurocholate-induced acute pancreatitis, presumably because of its detoxification activity against excessive ROS, and inhibits the activation of NF-κB by affecting IκB nitration and degradation. Our findings highlight the potential value of hydrogen-rich saline as a new therapeutic method on acute renal injury in severe acute pancreatitis clinically.
Highlights
Acute pancreatitis (AP) is an acute inflammatory disorder with variable severity ranging from self-limited to severe conditions
Some studies demonstrated that hydrogen-rich saline showed promising efficacy in animal models of several inflammatory diseases including AP
[13,14,15], but there are no reports of hydrogen-rich saline in treating severe acute pancreatitis (SAP) associated acute kidney injury and other extrapancreatic organs injury
Summary
Acute pancreatitis (AP) is an acute inflammatory disorder with variable severity ranging from self-limited to severe conditions It is about 20% of AP experience severe attacks, such as complicate with multiple-organ dysfunction syndrome frequently [1]. Acute renal failure is a major prognostic factor in SAP and is known to occur in the early stage of this disease. It could promote the failure of other organ systems and accelerate the progression of the disease, subsequently leading to death. There are no experimental studies about extrapancreatic organ injuries and few researches to explore the protective mechanisms of hydrogen-rich saline concretely for SAP. We designed this experiment to evaluate the effects of hydrogen-rich saline on acute renal injury in SAP and explore its possible mechanisms
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