Abstract
Rhodobacter sphaeroides O.U.001 was grown in media with different concentrations of molybdenum and iron to study the expression level of nifD and nifK genes coding for the large subunit of Mo-nitrogenase and hupS gene coding for the small subunit of uptake hydrogenase. Hydrogen productions under the same growth conditions were also evaluated. Increasing concentrations of the molybdenum and iron stimulated hydrogen production and the highest total hydrogen accumulation was achieved in 16.5 μ M sodium molybdate (0.84 l H 2/l culture at t = 168 h ) and 0.1 mM ferric citrate (1.14 l H 2/l culture at t = 240 h ) containing media. Maximal expressions of nifD and nifK were observed from the late log phase until the mid-stationary phase of growth and hupS expression was seen as soon as the hydrogen was produced in the cells. Moreover, nifK, nifD and hupS expressions were significantly reduced in the molybdenum and iron starved cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
