Hydrogen Peroxide Enhances Fatty Acid 2-Hydroxylase Expression to Impede the Lipopolysaccharides-Triggered Apoptosis of Human Mesenchymal Stem Cells

Abstract

As a type of stem cells that mainly exist in the connective tissue or interstitium, mesenchymal stem cells (MSCs) exhibit great potential in self-renewal and multi-directional differentiation. They have been clinically utilized for the treatment of various diseases including cancer. This study aims to provide solid evidence for the further development and application of MSCs in human diseases. MSCs were assigned into 5 groups: control group, LPS group, low-, medium- and high-dose hydrogen peroxide groups. After one-hour treatment with LPS, MSCs were exposed to H 2 O 2 for 12 hours followed by analysis of cell apoptosis, viability via EdU staining, TUNEL assay and flow cytometry, FA2H expression by qPCR and Western blotting. The hydrogen peroxide treatment reduced proportion of apoptotic cells induced by LPS, along with enhanced viability and milder DNA damage. In addition, hydrogen peroxide impeded the LPS-triggered apoptosis of human MSCs. The results above proved that hydrogen peroxide significantly impeded the LPS-triggered apoptosis of MSCs, and further increased cell viability. This protective effect of hydrogen peroxide was mainly achieved by upregulation of FA2H expression. In conclusion, hydrogen peroxide can enhance FA2H expression to impede the LPS-triggered apoptosis of human MSCs. This finding helps to improve the further development and application of MSCs in treating human diseases.