Abstract
Hydrogen peroxide (H2O2) detection in biological systems is of significant importance, which act as critical second messenger in fundamental biological processes. Here, we report on a chemoselective fluorescent naphthylimide peroxide probe (NPP) for the H2O2 detection in vitro and in vivo. NPP is a phenylboronic acid-caged chromophore that selectively responds to H2O2 through a self-immolate mechanism. NPP exhibited high sensitivity and selectivity to H2O2 with distinctive fluorescence change due to the excellent two-photon excitation property, which permits the facile detection of inflammation produced H2O2 and offers chance to monitor the inflammatory stages in diseased cells.
Highlights
Reactive oxygen species (ROS) play essential roles in cellular physiological activities as signal transduction molecules [1]
H2O2 detection in biological systems is of significant importance since it offers useful information to reflect the progression of many pathological events
We report a two-photon excited fluorescent H2O2 probe that could respond to H2O2 in physiological conditions in an easy-to-monitor manner with deep tissue penetration capability [18,19,20]
Summary
Reactive oxygen species (ROS) play essential roles in cellular physiological activities as signal transduction molecules [1]. In vitro and in vivo results show that the NPP is capable of specific detection on the inflammation produced H2O2 through two-photon imaging, offering chances to monitor the inflammatory stages in diseased cells. LPS-induced H2O2 produce and fluorescence imaging RAW 264.7 cells were seeded in U 20 mm glass bottom dishes (Corning, USA), and appropriate amount of LPS was added and incubated for the indicated time periods.
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